Generator

Part:BBa_K3222001

Designed by: Ryo NIWA   Group: iGEM19_GIFU_TOKAI   (2019-11-01)


sfGFP (Lack of 3' UTR)

Group: GIFU TOKAI 2019

Author: Ryo NIWA

Documentation:

This part was made to use as control for iGEM GIFU TOKAI 2019's project. Since this part does not have 3' UTR region, it is low-expression and you cannot confirm sfGFP as fluorescence. We confirmed this part is functional by performing Western Blotting. When this part is circularized, this part may be possible to produce high amount of sfGFP. The part was transcripted and the mRNA was put as linear RNA in cell-free system called PUREfrex. The result was shown below. Control DNA was BBa_ I746916 and prepared following the instruction of PUREfrex.

T--GIFU_TOKAI--western_last.png

Figure.1 Western Blotting


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 47


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